diff --git a/fastapi/dependencies/utils.py b/fastapi/dependencies/utils.py
index 7be8f774c7..8c2cc2a243 100644
--- a/fastapi/dependencies/utils.py
+++ b/fastapi/dependencies/utils.py
@@ -1058,6 +1058,15 @@ def _extract_form_items(
                 yield key, param_values, None
 
 
+async def _read_file_bytes(file: UploadFile) -> bytes:
+    # Automatic conversion must not consume a file shared with other parameters.
+    position = file.file.tell()
+    try:
+        return await file.read()
+    finally:
+        await file.seek(position)
+
+
 async def _extract_form_body(
     body_fields: list[ModelField],
     received_body: FormData,
@@ -1067,7 +1076,7 @@ async def _extract_form_body(
             if is_bytes_or_nonable_bytes_annotation(
                 field.field_info.annotation
             ) and isinstance(value, UploadFile):
-                value = await value.read()
+                value = await _read_file_bytes(value)
             elif is_bytes_sequence_annotation(
                 field.field_info.annotation
             ) and value_is_sequence(value):
@@ -1075,7 +1084,7 @@ async def _extract_form_body(
                 results: list[bytes | str] = []
                 for sub_value in value:
                     if isinstance(sub_value, UploadFile):
-                        results.append(await sub_value.read())
+                        results.append(await _read_file_bytes(sub_value))
                     else:
                         results.append(sub_value)
                 value = serialize_sequence_value(field=field, value=results)
diff --git a/tests/test_upload_file_read_position.py b/tests/test_upload_file_read_position.py
new file mode 100644
index 0000000000..8e8a8ec592
--- /dev/null
+++ b/tests/test_upload_file_read_position.py
@@ -0,0 +1,147 @@
+from io import BytesIO
+from typing import Annotated
+
+import pytest
+from fastapi import Depends, FastAPI, File, UploadFile
+from fastapi.dependencies.utils import _read_file_bytes
+from fastapi.testclient import TestClient
+
+
+@pytest.mark.parametrize(
+    "content",
+    [
+        pytest.param(b"hello", id="memory"),
+        pytest.param(b"x" * (1024 * 1024 + 1), id="disk"),
+    ],
+)
+def test_file_bytes_shared_by_dependency_and_endpoint(content: bytes):
+    app = FastAPI()
+
+    def inspect_file(file: Annotated[bytes, File()]):
+        return file.decode()
+
+    @app.post("/")
+    def upload(
+        inspected: Annotated[str, Depends(inspect_file)],
+        file: Annotated[bytes, File()],
+    ):
+        return {"dependency": inspected, "endpoint": file.decode()}
+
+    client = TestClient(app)
+    response = client.post("/", files={"file": ("hello.txt", content)})
+    assert response.status_code == 200
+    assert response.json() == {
+        "dependency": content.decode(),
+        "endpoint": content.decode(),
+    }
+
+
+def test_file_bytes_list_shared_by_dependency_and_endpoint():
+    app = FastAPI()
+
+    def inspect_files(files: Annotated[list[bytes], File()]):
+        return [file.decode() for file in files]
+
+    @app.post("/")
+    def upload(
+        inspected: Annotated[list[str], Depends(inspect_files)],
+        files: Annotated[list[bytes], File()],
+    ):
+        return {"dependency": inspected, "endpoint": [file.decode() for file in files]}
+
+    client = TestClient(app)
+    response = client.post(
+        "/",
+        files=[
+            ("files", ("one.txt", b"one")),
+            ("files", ("two.txt", b"two")),
+        ],
+    )
+    assert response.status_code == 200
+    assert response.json() == {
+        "dependency": ["one", "two"],
+        "endpoint": ["one", "two"],
+    }
+
+
+def test_bytes_dependency_preserves_upload_file_for_endpoint():
+    app = FastAPI()
+
+    def inspect_file(file: Annotated[bytes, File()]):
+        return file.decode()
+
+    @app.post("/")
+    async def upload(
+        inspected: Annotated[str, Depends(inspect_file)],
+        file: Annotated[UploadFile, File()],
+    ):
+        content = await file.read()
+        second_read = await file.read()
+        return {
+            "dependency": inspected,
+            "endpoint": content.decode(),
+            "second_read": second_read.decode(),
+        }
+
+    client = TestClient(app)
+    response = client.post("/", files={"file": ("hello.txt", b"hello")})
+    assert response.status_code == 200
+    assert response.json() == {
+        "dependency": "hello",
+        "endpoint": "hello",
+        "second_read": "",
+    }
+
+
+def test_automatic_file_read_preserves_nonzero_position():
+    app = FastAPI()
+
+    async def read_prefix(file: Annotated[UploadFile, File()]):
+        return (await file.read(2)).decode()
+
+    def inspect_remainder(
+        prefix: Annotated[str, Depends(read_prefix)],
+        file: Annotated[bytes, File()],
+    ):
+        return {"prefix": prefix, "remainder": file.decode()}
+
+    @app.post("/")
+    async def upload(
+        inspected: Annotated[dict[str, str], Depends(inspect_remainder)],
+        file: Annotated[UploadFile, File()],
+    ):
+        position = file.file.tell()
+        remainder = await file.read()
+        return {
+            "dependency": inspected,
+            "position": position,
+            "remainder": remainder.decode(),
+        }
+
+    client = TestClient(app)
+    response = client.post("/", files={"file": ("hello.txt", b"hello")})
+    assert response.status_code == 200
+    assert response.json() == {
+        "dependency": {"prefix": "he", "remainder": "llo"},
+        "position": 2,
+        "remainder": "llo",
+    }
+
+
+@pytest.mark.anyio
+async def test_failed_automatic_file_read_restores_position(
+    monkeypatch: pytest.MonkeyPatch,
+):
+    with BytesIO(b"hello") as stream:
+        file = UploadFile(file=stream)
+        stream.seek(2)
+
+        async def failing_read(size: int = -1) -> bytes:
+            stream.read(1)
+            raise OSError("read failed")
+
+        monkeypatch.setattr(file, "read", failing_read)
+        with pytest.raises(OSError, match="read failed"):
+            await _read_file_bytes(file)
+
+        assert stream.tell() == 2